Why were the leaf discs incubated at a constant temperature during the experiment?

The purpose of incubating the leaf discs at a constant temperature is fundamentally linked to maintaining a steady environment that supports the underlying physiological processes. When temperature varies, it can influence metabolic activities, including photosynthesis and respiration rates. By stabilizing the temperature, the experiment minimizes fluctuations that could affect these rates, thereby helping ensure that diffusion gradients are consistent.

Additionally, a constant temperature helps ensure that the rate of gas exchange remains uniform across all samples. This control is crucial because variations in temperature could lead to different levels of enzyme activity and, consequently, differences in the rates of photosynthesis that might skew the results. Maintaining temperature consistency, therefore, allows for more accurate and reliable comparisons between the leaf discs during the experiment.